38.
Michaelis and Menten derived their equation in enzyme kinetics using which of the following assumptions ?
A:
Rate limiting step in the reaction is the breakdown of ES complex to product and the free enzyme
B:
Rate limiting step in the reaction is the formation of ES complex
C:
Concentration of the substrate can be ignored
D:
Non – enzymatic degradation of the substrate is the major step
The answer is:
A