M.Sc. Bio-Physics (PU-CET P.G) 2016 Panjab University Entrance Exam With Answers

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44.

The most important property of any microscope is its power of resolution, which is numerically equivalent to D, the minimum distance between two distinguishable objects. D depends on three parameters, namely, the angular aperture, α, the refractive index, N, and the wavelength, λ, of the incident light. Below are given few possible options to increase the resolution of the microscope.

P. Decrease the value of λ or increase either N or α to improve the resolution
Q. Moving the objective lens closer to the specimen will decrease sin α and improve the resolution
R. Using a medium with high refractive index between the specimen and the objective lens to improve the resolution
S. Increase the wavelength of the incident light to improve the resolution
Which of the following combination of the above statements is correct ?
A: P and R
B: Q and R
C: P and S
D: R and S

The answer is: A