Bio-Physics (M.Phil./Ph.D.) 2015 Panjab University Entrance Exam With Answers

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16.

Michaelix and Menten derived their equation on enzyme kinetics using which of the following assumption -

A: Rate limiting step in the reaction is the breakdown of ES complex to product and free enzyme
B: Rate limiting step in the reaction is the formation of ES complex
C: Concentration of the substrate can be ignored
D: Non-enzymatic degradation of the substrate is the major step

The answer is: A