Biotechnology Engineering (M.Phil./Ph.D.) 2016 Panjab University Entrance Exam With Answers

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11.

An enzyme shows highest activity in the pH range 2.0 - 3.0. At pH 4.0 and pH 7.0, the enzyme exhibits 50% and 1%, respectively, of its highest activity. Which of the following states of anamino acid residue in the catalytic site is most responsible for its activity profile?

A: A protonated Asp
B: A deprotonated Asp
C: A deprotonated Asn
D: A protonated Asn

The answer is: A