M.Sc. Bio-Chemistry (PU-CET P.G) 2017 Panjab University Entrance Exam With Answers

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32.

The cell-free extract prepared from E. coli cells over-expressing enzyme β- glucosidase showed the activity of 1.5 units per ml (protein concentration 2 mg per ml). The Ni-NTA purified preparation showed the activity of 75 units per ml (protein concentration 100 μg per ml). Calculate the fold purification of the enzyme achieved?

A: 0.001
B: 0.02
C: 50
D: 1000

The answer is: D