Biotechnology Engineering (M.Phil./Ph.D.) 2016 Panjab University Entrance Exam With Answers

Prepare for Biotechnology Engineering M.Phil./Ph.D. Entrance Exams at Panjab University with Jetty Study. Access previous year papers in MCQ format with answers.

1. The release of Ca^2+ from endoplasmic reticulum to cytoplasm in response to stimulus is mediated by

2. Which one of the following 0.1M solution has the lowest pH

3. Shine-Delgarno sequence is a part of

4. Cori cycle integrates body metabolism to

5. Blood group antigens are

6. if 1 mL of a solution of 0.01 M HCl is diluted to 100 mL at 25°C, the pH of the resulting solution will be

7. Chromosome constitution in case of Turner’s syndrome is

8. A person with increased interferon levels in his serum is likely to be suffering from

9. For glycolipoproteins, most commonly used probe

10. Bt toxin, produced by Bacillus thuringiensis, does NOT kill the bacteria itself because the toxin is

11. An enzyme shows highest activity in the pH range 2.0 - 3.0. At pH 4.0 and pH 7.0, the enzyme exhibits 50% and 1%, respectively, of its highest activity. Which of the following states of anamino acid residue in the catalytic site is most responsible for its activity profile?

12. The agglutinin test is used for

13. Cephalin, a biological surfactant, is

14. N and N0 represent the number of viable cells at time ‘t’ during sterilization and at the start of sterilization (t=0), respectively. Assuming that cell death follows first order kinetics and that k is the death rate constant, which of the following relationship(s) is/are correct? kt

15. In an experiment conducted in the dark, isolated chloroplasts are kept in buffer (pH 4.0) at 4°C until their internal pH is equal to 4.0. Then, they are transferred to a buffer of pH 8.0, and ADP and Pi are added at the same time. Which of the following will happen?

16. Two mammalian cell lines with doubling times of 12 h and 36 h were cultured with radioactive thymidine for 8 h. The cells were further cultured without the radioactive thymidine for 72 h. Incorporated radioactivity was measured in equal number of cells in each culture, which revealed that

17. The osmotic pressure of a solution containing 1.71 gm of sucrose (mol. wt. 342) dissolved to make a litre of solution at 27°C (R = 0.082 LatmK-1 mol-1) is ?

18. Given are the sequences of one strand of double-stranded DNA. The one with the highest melting point (Tm) is

19. The preferred system for large-scale production of influenza virus for vaccination is

20. X-ray diffraction of wool shows repeated structural units spaced at 5.2 Å, which is changed to7.0 Å on steaming. This is due to the conversion of secondary structure from

21. Vasopressin, an antidiuretic hormone, responsible for increased absorption of water by thekidney, is secreted from

22. Helicobacter pylori can survive in the highly acidic environment of human stomach because it

23. Pfu polymerase has

24. The molar abosorptivity of a1×10 M ATP solution, which has an absorbance of 0.20 and pathlength is 2.5 cm

25. At Et = 20 nm and substrate concentration = 40 μM, the reaction velocity Vo of an -I -1 enzyme is 9.6 μMs . Assuming kcat to be 600 s , the KM will be

26. A peptide Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly, having an isoelectric point of 7.8, isplaced in an electric field at pH 3.0. It will migrate towards

27. Three amino acids of molecular weights 75, 89 and 105 Dalton each have a tripeptide. The molecular weight of tripeptide in Dalton will be

28. Lysine is an amino acid with three ionizable groups. These are the α-COOH, α-amino and ε-amino groups with pKa values of 2.2, 9.2 and 10.8, respectively. The isoelectric point (pI) for the lysine is

29. In a dihybrid test-cross, 200 recombinant phenotypes were observed among 2402 progeny. The distance between two genes is

30. A phage infects bacteria at a multiplicity of infection (moi) of 0.1. This means that

31. A disease manifests only in the homozygous recessive condition. A couple has two children. If both parents are heterozygous for the disease causing gene, what is the probability that both the children are normal? Assume that the disease causing gene is not sex linked

32. A bacterial culture contained 32x10^6 cells after 2.5 hours of exponential growth. If the doubling time was 30 min, what was the initial population number in this culture ?

33. When a number of genes are transcribed as one mRNA, such mRNA is termed as

34. Which one of the following is NOT true of RNA polymerase II?

35. Choose the option that shows the correct pairing of the products with their corresponding microorganisms.

36. Choose the option that lists the correct sequence of steps involved in gene therapy.

37. Match the techniques in column I with their primary applications in Column II:

38. The most preferred substrate for conversion into methane by methanogens is:

39. How many moles of biodiesel are obtained from 1 mole of triolein

40. What is the triglyceride and alcohol operating ratio generally used in transesterification reaction used for biodiesel production

41. How much glucose can be obtained upon the hydrolysis of 1 mole of cellulose

42. How much ethanol can be obtained upon fermentation with Saccharomyces cerevisisae from 1 mole of glucose

43. How many ATP molecules are produced upon fermentation of 1 mole of glucose by Zymomonas mobilis

44. How many moles of ethanol can be produced upon the fermentation of 1 mole of xylose

45. Urea degrading bacteria is

46. Contribution of CO2 in the carbon cycle is by

47. Nitrogen cycle has linkage with

48. Which of the following is the most efficient biofilteration in the secondary treatment of the sewage?

49. Pasteurization involves the