During SDS-PAGE, the separation of protein is based on their molecular masses rather than charge. Which of the following contributes to such an effect.

M.Sc. Bio-Chemistry (PU-CET P.G) 2019 Panjab University Entrance Exam With Answers

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37.

A: SDS to protein binding ratio of 1.4 g SDS per gram of Protein

B: SDS lend net positive charge on all the proteins

C: SDS causes denaturation of proteins so all proteins become neutral

D: SDS hydrolyzes proteins in stack of low to high molecular weight proteins

The answer is: A

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