Biotechnology Engineering (M.Phil./Ph.D.) 2014 Panjab University Entrance Exam With Answers

Practice Mode:
34.

A novel sea organism was identified giving blue luminiscense. For observing the true expression of the gene responsible for this character which of the following strategies will you follow ?

A: Isolate the genomic DNA from the organism and directly injecting to bacteria, screen colonies for bioluminescence.
B: Isolate the genomic DNA from the organism, treat with DNAsel, followed by restriction endonuclease treatments and transform into eukaryotic cell such as yeast, screen colonies for bioluminescence.
C: Isolate the mRNA from the organism, reverse transcribe and generate cDNA, transform the virus and screen colonies for bioluminescence.
D: Isolate the mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into eukaryotic cells such as yeast screen, colonies for bioluminescence.

The answer is: D

<< Previous Question
Next Question >>