Biotechnology Engineering (M.Phil./Ph.D.) 2019 Panjab University Entrance Exam With Answers

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1.

During each cycle of PCR the reaction mixture is transferred between three temperatures. All the following statements are true for these temperatures, except;

A: The denaturation temperature helps to release the single stranded DNA to act as templates
B: The hybridization temperature facilitates primers to attach to the templates.
C: Annealing temperature is the one at which DNA synthesis occurs.
D: The extension temperature is usually set just below the optimum temperature of Taq polymerase.

The answer is: C

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