Bio-Chemistry (M.Phil./Ph.D.) 2014 Panjab University Entrance Exam With Answers

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What is the starting point for selection of a suitable Ion-exchange chromatography matrix for purification of a recombinant proteins?

A: Prediction of isoelectric point (pl) from the amino acid sequence
B: Test proteins binding to an Ion-exchange matrix at a range of pHs and salt concentrations
C: Test protein binding to a selection of anion and cation exchange matrices
D: Pass your sample through a preparative column and elute with a salt gradient

The answer is: A