Bio-Chemistry (M.Phil./Ph.D.) 2016 Panjab University Entrance Exam With Answers

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11.

Absorbance spectra values of a DNA sample at wavelength 210, 225, 260, 277, 280 and 290 were recorded as 1, 1.2, 2.0, 1.4, 1.5 and 1.45. What would be the quantity and quality of the given DNA sample (given 1.0 OD at A260 =50μg/ml DNA amount)

A: 50 μg/ml, contaminated with protein
B: 100 μg/ml, contaminated with protein
C: 50 μg/ml, DNA free of protein contamination
D: 100 μg/ml, DNA free of protein contamination

The answer is: B