You have to determine the amino acid sequence of a peptide. You perform the following steps using enzyme cleavage of your peptide (see table below):
Step 1: Treatment with trypsin yields three fragments with the following sequences (in the order of their length): Trp-Gly-Ala , Ala-Gly-Thr-Lys, Tyr-Leu-Asp-Arg.Step 2: Treatment with chymotrypsin gave the following three peptide fragments: Gly- Ala, Leu-Asp-Arg-Trp, Ala-Gly-Thr-Lys-Tyr. What is the sequence of your peptide?