Biotechnology Engineering (M.Phil./Ph.D.) 2019 Panjab University Entrance Exam With Answers

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38.

You are asked to clone a plant gene using Agrobacterium tumifaciens. You are asked to use binary vector for this cloning process. Which of the following vectors you would chose for this experiment.

A: Vector that has vir genes in separate vector and Ti DNA replaced with target genes in other vector
B: Vector that has all the components of Ti plasmid
C: Vector that has vir genes and T-DNA together on one vector and target gene in other vector
D: Vector that has vir genes and target genes in separate vector and Ti DNA in other vector

The answer is: A