Biotechnology Engineering (M.Phil./Ph.D.) 2017 Panjab University Entrance Exam With Answers

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5.

Upon purification of an unknown protein from yeast, the native gel electrophoresis showed the a single band corresponding to molecular weight of 100 kDa. When you separated this protein employing SDS-PAGE, you gave final inference that this unknown protein is a homodimer, Which of the following result of SDS-PAGE led to this inference of yours ?

A: Appearance of a single band of 30 kDa
B: Appearance of a two bands of 100 kDa
C: Appearance of single band of 100 kDa
D: Appearance of two bands one at 30 kDa and the other at 100 kDa

The answer is: A