A reporter gene construct consisted of NFAT responsive promoter linked to a green fluorescence protein (GEP) gene fragment and the construct was transfected to animal cells in culture. Treatment of these cells with X compound led to expression of GEP and the cells looked green. Which of the following led to such an effect?

Biotechnology Engineering (M.Phil./Ph.D.) 2017 Panjab University Entrance Exam With Answers

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A: Compound X signals to hydrolyze the NFAT promoter region to release GFP for giving green colour

B: Compound X acted as antisense to blocked NFAT expression hence only GFP gets expressed and gives green colour

C: Compound X signals to synthesize aromatic amino acid rich protein that gave overall green colour to the cells

D: Compound X signals to translocate cells native NFAT to Nucleus leading to expression of GEP to given green colour

The answer is: D

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